Methods: Extracted Venenum Bufonis remains was determined by physical and chemical test and TLC.

方法：采用理化鉴别和薄层色谱鉴别法对提取后的蟾酥残渣进行检查。

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Results: The optimum extraction technology of Venenum Bufonis is using 90% and 80% alcohol extraction.

结果：蟾酥最佳提取工艺为采用90 %、80 %乙醇依次提取。

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Results Venenum Bufonis had growth inhibitory and apoptosis-inducing effects on U2OS cell with a time and dose dependent relation.

结果蟾酥水提取物对U2 OS细胞具有生长抑制及诱导凋亡作用，且呈时间依赖和剂量依赖。

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