The sequencing of highly conserved 5' UTR of the Chinese HGV strain has important implication for the development of genetic diagnostic assays.

HGV高度保守区5＇UTR的分子克隆与核苷酸序列的测定对开展HGV感染的基因诊断具有重要意义。

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Objective; To construct a promoter identifying plasmid with GFP as reporter gene, and then identify the promoter activity of HCV 5 '- UTR with this construct.

目的：利用已有质粒构建带GF P报告基因的启动子鉴定质粒，并用此质粒鉴定丙型肝炎病毒5非翻译区(HCV 5 ' - UTR)启动基因表达的活性。

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Results it was found by sequence contrast that some mutation sites occurred mainly on UTR region, and a lot of protein sequences changed according to changes of genes coding region.

结果经序列比对发现突变位点较多发生在U TR区域，同时一些基因的编码区域也发生蛋白序列的变化。

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