The experimental results of urea gradient electrophoresis show that there are two different refolding processes.
尿素浓度梯度电泳实验结果显示，存在两条不同的折叠途径。
After denaturing in urea, gradient dialysis and cleavage of GST-tag, refolded human EGFR-TKD was obtained.
经尿素变性、梯度透析复性及去除GST“标签”后，获得重折叠的人EGFR胞内酪氨酸激酶结构域蛋白。
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