Conclusions The undecalcified bone sections of human made in the method can satisfy the need of IH and ISH.
结论低温塑料包埋方法制备的人类不脱钙骨组织切片可同时进行免疫组化和原位杂交的检测观察。
Objective To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.
目的：探讨在低温塑料包埋制备的不脱钙骨切片上运用免疫组化和原位杂交技术的可行性。
Animals were killed at 4 and 12 weeks after implantation surgery and undecalcified sections were prepared and examined histologically and histomorphometrically.
种植术后第4、12周分2批处死动物，制作不脱钙标本切片，形态学观察及骨计量学检测。
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