Methods:1. Low density lipoprotein was prepared by recipetation,Foam cell model was established by incubating U937 cells with 80mg/L oxidized low density lipoprotein for 48 hours.
方法:1.采用改良沉淀法提取人血浆低密度脂蛋白（low density lipoprotein,LDL）,硫酸铜氧化制备ox-LDL,与U937单核细胞共同孵育,建立泡沫细胞模型。
参考来源 - U937泡沫细胞中组织因子途径抑制物2的表达及变化规律的实验研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Methods Human monocytic U937 cells were used as a model of monocytes.
方法单核细胞的研究模型为人前单核细胞系u937细胞。
U937 cells mature and differentiate in response to a number of soluble stimuli, …
U937细胞会因为一些可溶性刺激物而成熟和异化，。
Objective to optimize the selective method to obtain the pure recombinant plasmid transfected U937 cells.
目的：探索优化重组质粒转染细胞后的筛选方法，以得到高纯度U 937转染细胞。
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