Method Trypsinase,neutrase,papain,pepsin and alkaline protease were used to hydrolyze soybean protein and to prepare soybean polypeptide with ACE inhibitive activity. The optimal condition of enzymatic hydrolysis was studied.
方法利用胰蛋白酶、中性蛋白酶、木瓜蛋白酶、胃蛋白酶和碱性蛋白酶分别水解大豆蛋白,制备具有ACE抑制活性的大豆多肽,通过正交优化试验确定碱性蛋白酶的最佳水解条件,考察底物浓度、反应时间、反应温度与大豆蛋白酶解多肽对ACE抑制活性的影响,并将超滤法和聚丙烯酰胺凝胺电泳技术相结合,考察大豆蛋白酶水解物的分子量分布。
参考来源 - 碱性蛋白酶水解大豆多肽及抑制ACE效果的研究The paper confirms enzyme for Gln active peptide hydrolization, trypsinase, alkaline proteinase, and optimization enzymolysis condition in the duration.
确定了Gln活性肽的水解用酶为胰蛋白酶和碱性蛋白酶。
参考来源 - 麦谷蛋白源谷氨酰胺肽的制备和肠道营养效果研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Extraction of Mucopolysaccharides from shark fin cartilage by digested with trypsinase was studied.
对胰蛋白酶水解鲨鱼鳍软骨提取粘多糖进行了较系统的研究。
The aim of this study was to observe the influences of hydrochloric acid, pepsin and trypsinase on vigor of Eperythrozoon, and explore its transmission in alimentary tract.
目的观察盐酸、胃蛋白酶、胰蛋白酶对附红细胞体活力的影响，探讨附红细胞体的消化道传播。
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