瞬时转染(transient transfection)指的是转染的核酸不整合到染色体上,结果是短暂的高水平表达,可在24—96小时内检测表达效果,表达水平与位置无关,不会受到周围...
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The vectors were identified by detecting the expression of reporter genes after transient transfection into eucaryotic cells and microinjection into mouse fertilized ovum.
将载体瞬时转染真核细胞和显微注射入小鼠受精卵后,检测报告基因的表达以鉴定载体活性。
参考来源 - 眼皮肤白化病的基因诊断/细胞分裂周期相关基因8(CDCA8)启动子及其转录因子的初步研究In addition, by transient transfection, we obtained SMMC-7721 cell clones supporting stably expression of HCV proteins.
另外我们通过瞬时转染筛选得到了可以稳定表达HCV蛋白的人肝癌细胞系SMMC-7721细胞克隆。
参考来源 - HCV结构蛋白在昆虫细胞和哺乳动物细胞中的表达及稳定转染HCV全基因组的SMMC·2,447,543篇论文数据,部分数据来源于NoteExpress
Objective: To optimize the condition of gene transient transfection in CHO-K1 cell.
目的:以CHO - K1细胞为宿主基因瞬时转染条件的优化。
Methods Transient and stable transfection experiments in cells and assays of chloramphenicol acetyltransferase activity and aromatase activity were used.
方法细胞瞬时和稳定转染及氯霉素酰基转移酶和芳香族化酶活性测定。
The recombinant vector was transfected into HEK293 cells with liposome transfection reagents for transient expression.
脂质体法转染HEK293细胞,用倒置荧光显微镜检测、分析其在HEK293细胞表达及定位。
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