At present, inbred lines of transgenic insect resistant maize with selective marker gene removed are in separating and inbreeding program assisted by DNA marker detection.
目前,正结合进行田间分离纯合和DNA分子鉴定,培育去除选择标记基因的转基因抗虫玉米自交系。
CBF1 gene from rape was transformed into Arabidopsis thaliana by Agrobacterium tumefaciens-mediated method to screen its transgenic plants with resistance and conduct PCR detection and GUS staining.
采用根癌农杆菌介导法将油菜CBF1基因转入拟南芥,筛选抗性转基因拟南芥植株,进行PCR检测和GUS染色。
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