论生长素信号对植物生长与系统性获得抗性的调控作用-期刊文摘-论文中国 、侧根的起始生成、向地性和向光性等。同时,在分子水平上,生长素还调控着无数基因的表达。近年来的研究,尤其是生长素受体转运抑制响应蛋白(transport inhibitor response protein 1,TIR1)和疑似受体生长素结合蛋白(auxin binding protein 1,ABP1)的发现,使得生长
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By filling in a hydrophobic cavity at the protein interface, auxin enhances the tir1-substrate interactions by acting as a 'molecular glue'.
通过填充tir 1蛋白表面疏水性空腔，植物生长素在增强TIR1 -底物相互影响中起着“分子胶”作用。
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.
将构建好的ABP1及TIR1原核表达载体，转化表达宿主菌bl21 (DE3)，加入诱导物iptg进行诱导表达。
In this study, we found that expression form of ABP1 was soluble so we purified it in natural conditions, at the same time, the TIR1 was purified in inclusion body conditions.
针对两种蛋白的不同表达形式，ABP1蛋白采用天然条件下纯化的方式，而TIR1蛋白采用包涵体纯化的方式。
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