论生长素信号对植物生长与系统性获得抗性的调控作用-期刊文摘-论文中国 、侧根的起始生成、向地性和向光性等。同时,在分子水平上,生长素还调控着无数基因的表达。近年来的研究,尤其是生长素受体转运抑制响应蛋白(transport inhibitor response protein 1,TIR1)和疑似受体生长素结合蛋白(auxin binding protein 1,ABP1)的发现,使得生长
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Anchored to the base of the TIR1 pocket, auxin binds to a partially promiscuous site, which can also accommodate various auxin analogues.
与TIR 1结合区的碱基连接后，植物生长素绑定到局部杂乱结合域，该结合域也能容纳不同的生长素类似物。
By filling in a hydrophobic cavity at the protein interface, auxin enhances the tir1-substrate interactions by acting as a 'molecular glue'.
通过填充tir 1蛋白表面疏水性空腔，植物生长素在增强TIR1 -底物相互影响中起着“分子胶”作用。
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.
将构建好的ABP1及TIR1原核表达载体，转化表达宿主菌bl21 (DE3)，加入诱导物iptg进行诱导表达。
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