1．2基因打靶的操作 1．2．1基因打靶载体的构建 进行基因打靶，关键在于打靶载体（targeting vector）的构建。要构建打靶载 体，最重要的就是同源的DNA序列，就是目的基因两端含有与靶基因序列相同的 核苷酸序列，即基因打靶序列。
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Gene targeting vector 基因打靶载体
targeting-vector 打靶载体
a targeting vector 定点打靶载体
hrDNA targeting vector 人核糖体DNA打靶载体
universal gene targeting vector 通用型基因打靶载体
human ribosomal DNA-targeting vector 核糖体基因区打靶载体
Targeting retroviral vector 靶向性逆转录病毒载体
Conclusion A conditional gene targeting vector of mouse Bpi has been successfully constructed.
结论成功构建了小鼠Bpi条件基因打靶载体。
参考来源 - 小鼠Bpi条件基因打靶载体的构建和鉴定Ⅱ: Phage positive clone P14 could specifically target to colon cancer LoVo cells;it may become the targeting vector to restrain colon cancer and can be further used to the development of targeting therapeutic agents against colon cancer in the future.
Ⅱ:本研究鉴定了P14噬菌体克隆能与大肠癌LoVo细胞特异性结合,它有可能成为抑制大肠癌的靶向载体,为进一步研制大肠癌特异性靶向治疗药物奠定了初步的实验基础。
参考来源 - 噬菌体展示肽库筛选大肠癌细胞特异性结合肽的实验研究·2,447,543篇论文数据，部分数据来源于NoteExpress
Conclusion The mouse EMSP1 gene targeting vector is constructed successfully.
结论：成功构建小鼠emsp1基因打靶载体。
Vector retargeting is one of the important strategies on viral vector targeting.
载体的靶向策略很多，载体重靶向就是其中重要的一种。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
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