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Methods: The pain threshold(PT) was determined by radiant heat-induced tail flick test.
方法：采用辐射热甩尾法测定大鼠痛阈变化；
METHODS the writhing model of mice induced by acetic acid, hot plate test (sc, icv), tail flick test and the model of electrical stimulation induced contraction of guinea pig ileum were used.
方法通过醋酸扭体模型、热板、甩尾、纳洛酮拮抗试验及豚鼠回肠离体标本研究样品的镇痛活性及机制。
Meanwhile, carry on the animal behavior test, including Open Field behavior, Hole-board test, Tail Flick Latency (TFL) test and Step-down test to discover the gene function on the whole animal level.
同时进行旷场行为测试、洞板试验、甩尾试验、步下法试验等动物行为学实验，旨在研究这些基因在整体动物水平上的功能表现。
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