Spleen lymphocyte transforming index was measured with MTT method, and subtypes of t lymphocyte were counted by immunofluorescence to observe the influence on the immunological function.
应用MTT法测定脾淋巴细胞转化指数。用免疫荧光法测定脾t淋巴细胞亚群细胞数,观察其对免疫功能的影响。
Methods t lymphoid subgroups and the percentage of NK cells were detected by the use of indirect immunofluorescence method the function of NK cells was detected by MTT.
方法采用间接免疫荧光法检测白血病患者T淋巴细胞亚群、NK细胞百分数,MTT法检测NK细胞杀伤活性。
The killing activity of NK cells and the proliferation rate of spleen cells were tested by MTT assay, and T cell subgroupCD4+ , CD8+ were tested by the indirect immune fluorescent assay.
用MTT方法对脾脏的NK杀伤细胞率和淋巴细胞的转化率进行测定,采用免疫荧光法对CD4+、CD8+细胞进行测定。
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