Conclusion: Regions of apoptotic cells in "API method" and sub-G1 method were not the same. There is no coincidence in results of apoptotic percentage detected by "API method" and sub-G1 method.
结论:“API技术”和亚g 1峰检测法的细胞凋亡域不一致,“API技术”对细胞周期特异性凋亡的检测结果与亚g 1峰检测结果也不一致。
Results: (1) Sub-confluent cells having starved in DMEM containing 0.4% FBS for 48h, 88.5% of the cells were at G0/G1 phase of the cell cycle.
研究结果表明:(1)培养至次融汇状态的乳鼠心脏成纤维细胞经低血清(0.4%FBS) 饥饿培养48小时,G0/G1期细胞占88.5%;
HL 60 and K562 cells showed typical apoptosis features of "ladder" and the sub G1 peak in DNA histogram.
作用HL 60和K562细胞后电泳显示有凋亡细胞特有的“梯状”条带,DNA直方图出现亚g1峰。
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