The retention volume of targeted compounds was predicted in the step gradient elution conditions.
预测了台阶梯度洗脱条件下待分离物质的保留体积。
The gradient should already be set to the same one we created in the previous step.
渐变应该已经被自动设置为我们前一步使用的参数（你需要做相应修改——译者注）。
This step is optional, but I created a layer and have applied a linear gradient from top to bottom to increase the contrast at the bottom of the PIC.
这一步是可选的，但我还是建了一层来应用一个从上到下的渐变来增强图像底部的对比度。
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