AIM: to explore RP-HPLC with single-step extraction to determine the concentration of dexamethasone acetate (DA) in vitreous body of rat eyes.
目的：探讨单步萃取—反相高效液相色谱法(RP - HPLC)测定大鼠眼玻璃体腔醋酸地塞米松(DA)浓度的可行性。
Conclusion: the application of "four-step extraction" method enhances the peptide recovery during in-gel digestion, and increases identification of the protein.
结论：应用“四步提取法”使得酶解肽段提取率和蛋白质的有效鉴定率明显提高。
Two-step extraction with polar solvents and nonpolar solvents was an effective comprehensive extraction for antioxidant and essential oil of rosemary Rosmarinus officinalis l.
极性溶剂和非极性溶剂的两步提取法是迷迭香抗氧化剂和精油综合提取的有效方法，操作简单，容易实施。
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