Conclusion This study suggests a valid and safe method that can be used for efficient passage of neural stem cells.

结论消化振荡法对人胚胎神经干细胞的活性影响较小，是一种有效、安全的将神经球打散成单细胞悬液的实验方法。

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Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.

方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。

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The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.

方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。

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We're also continuing to make progress on several other fronts-- providing vital preventive health care services for women through the Affordable Care Act, furthering STEM education for girls, and advocating for the passage of the Paycheck Fairness Act.

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