孢子分离法 在无菌操作条件下使孢子在适宜的培养基上萌发，长成菌丝体而获得纯菌种 的方法称为孢子分离法（spore isolation）。其特点是：菌丝菌龄短，因时有性繁 殖产物，其菌丝生命力强。
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Spore Isolation Strains 孢子分离菌株
the single spore isolation 单孢分离
multi-spore isolation strains 多孢分离菌株
Isolation breeding of spore 孢子分离育种
Single spore isolation analysis indicated that 35% genotypic segregation was obtained from heterokaryotic strain.
单孢分离分析表明，异核体的分离频率约为35%。
The whole isolation procedure only involved in some simple instruments and single spore was directly isolated from culture or disease sample under microscopic field using dissecting needle.
该法只需利用简单的仪器和用具，从培养物或标样上挑取孢子，直接在显微镜下分离单孢的方法。
This paper reported isolation and regeneration of the dikaryocyte protoplasts from Pleurotus sapidus, and the protoplasts were treated by U. V-irradiation for selection spore less mutants.
用紫外线照射紫孢侧耳（Pleurotus sapidus）双核菌丝原生质体，再生后筛选生长势好的菌株， 通过出菇试验得到了生产性状与紫孢侧耳一致的无孢和少孢平菇新品种。
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