With the stress degree stronger and the stress time longer, the protein in xylem sap changed among 14.4~31KD for all alternative split-root osmotic stress.
随着渗透胁迫强度的增加和胁迫时间的延长，木质部汁液中的可溶性蛋白质组分主要在14.4~31KD范围内变化。
Some new type of two-hybrid systems, such as split-ubiquitin system, protein-fragment complementation assay, repressor reconstitution assay and SOS recruitment system, have been developed recently.
前言： 近年来，一些不依赖于转录因子活性的新型双杂交系统相继建立，如分离的泛素系统、 蛋白质片段互补分析、 阻遏物重构分析和SOS恢复系统等。
This will help us to investigate controllable protein cleavage in vitro, further establish efficient protein purification system and investigate splicing mechanism of split-inteins.
这将为进一步研究其体外可控性剪接、构建高效的蛋白纯化系统和深入研究蛋白质内含子的剪接机制提供基础。
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