Aim: To evaluate the clinical value of MSP (methylation specific PCR) in detection of tumor suppressor genes methylation in the colorectal cancer.

目的：评价特异性甲基化pcr (msp)分析法检测抑癌基因高甲基化对结、直肠癌的诊疗价值。

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Objective To establish the methylation-specific PCR(MSP) to detect methylation of GTPase regulator associated with the focal adhesion kinase(GRAF) gene promoter.

目的建立甲基化特异性PCR（MSP）技术检测黏着斑激酶相关性鸟苷三磷酸酶调节因子（GRAF）基因启动子甲基化。

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