T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

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If men don't struggle, will be like the story that said, I sp6 only looking at you for another man continually give up their principles and the bottom line.

如果男人不奋斗，就会像故事三里所说的那样，我只能看着你为了另一个男人不断地放弃自己的原则和底线。

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