The artificial damage of the skeletal muscle during the preparation of single cell suspension could cause the earlier degradation of the DNA.
骨骼肌在制成单细胞悬液过程中的人为损伤,可造成DNA提前降解。
Place the spleen in a Petri plate with 20 ml of RPMI 10% FBS, cut in two pieces and squeeze each of them between two sterile glass slides to obtain a single cell suspension.
脾脏放置在含有细胞培养用的20毫升10%胎牛血清培养皿里,剪成两瓣,用无菌玻璃的边缘挤压,获得单细胞悬液。
Conditioned medium (CM) stemming from cell suspension culture could remarkably promote the formation of single cell clone from culture cells of Panax ginseng below a critical cell plating density.
来自细胞悬浮培养物的条件培养基能显著地促进人参培养细胞的单细胞在较低细胞植板密度培养时的克隆形成。
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