Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
PCR and single strand conformation polymorphism analysis (SSCP) were combined with DNA sequencing confirmation to screen all 28 exons of SCN5A gene.
采用PCR 单链构象多态性技术（SSCP）结合DNA序列测定证实，对病人SCN5A的全部2 8个外显子进行突变检测。
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