The homologous recombination vector pMADΔnos was constructed by the ligating the upstream and downstream sequences of nos acquired by PCR, and the spectinomycin resistance gene into shuttle vector pMAD.
同源重组载体pMADΔnos的构建是将PCR获得的nos基因上下游同源序列和壮观霉素抗性基因连入穿梭载体pMAD。
参考来源 - 一氧化氮对金黄色葡萄球菌生物膜影响的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
以上来源于: WordNet
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