Objective: Adenovirus AdEasy system is a good method for rapid constructing recombinant adenovirus by employing homologous recombination between recombinant shuttle plasmid carrying interesting gene and adenovirus backbone plasmid in prokaryocyte.
目的 腺病毒AdEasy系统是一种利用原核细胞完成重组穿梭质粒与腺病毒骨架质粒之间的同源重组，快速构建重组腺病毒的好方法。
参考来源 - 以Adeasy系统构建重组腺病毒的方法学改进METHODS: IFNγ cDNA was amplified by reverse transcription-polymerase chain reaction (RT-PCR) from human peripheral blood mononuclear cells, and cloned into adenoviral shuttle plasmid pShuttle to construct recombinant adenovirus carrying human IFNγ cDNA (Ad-IFNγ) by in vitro ligation.
方法:用逆转录聚合酶链反应(reversetranscription-polymerasechainreaction,RT-PCR)从人外周血单个核细胞中扩增出IFNγ的cDNA全长序列,插入腺病毒穿梭质粒pShuttle,经酶切和DNA测序鉴定,然后用体外连接法构建重组腺病毒Ad-IFNγ。
参考来源 - 体外连接法构建重组人γ干扰素腺病毒并检测其在真核细胞中的表达·2,447,543篇论文数据，部分数据来源于NoteExpress
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