Objective:To evaluate and analyze the effect of two kinds of nucleic acid extraction methods on quantitative determination of serum HBV-DNA.
目的:评价、分析两种不同核酸提取方法对血清HBV-DNA定量测定的影响。
参考来源 - 低浓度血清HBV·2,447,543篇论文数据，部分数据来源于NoteExpress
Objective It is to discuss the relation between the serum HBV-DNA content and the serological marker of HBV (HBV-M).
目的探讨乙型肝炎患者血清中HBV -DNA含量与HBV血清学标志物（HBV -M）的关系。
Objective The serum HBV-DNA has been extensively detected by fluorescence-quantitative real time PCR (FQ-PCR) in our country.
目的目前国内较多的医院采用荧光定量的方法对血清中的HBV - DNA进行定量检测。
Methods an Alkaline lysis method was used to isolate serum HBV-DNA for fluorescence quantitative PCR and compared with conventional phenol-chloroform extraction method and boiling lysis method.
方法使用碱裂解法、酚氯仿法和煮沸裂解法同时提取血清hbv - DNA，比较荧光定量pcr测定的重复性和测定值的差异。
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