测序反应（Sequencing Reaction） 在一新 PCR 板中加入： Big Dye V3.1（ABI） 1.0μl Big Dye buffer 1.0ul 10mM PCR 正向引物 0.3μl 蒸馏水 0.2μl 上一步经纯...
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Because the droplets are so small, they require much smaller volumes of the chemicals used in the sequencing reaction than do current technologies.
由于水滴这么小，在测序反应中就需要比现在技术使用的化学品更小的体积。
The effects of DNA templates, primers, cycle sequencing reaction conditions, purification methods, operation of instrument were comparatively analyzed.
对测序中的模板、引物、测序反应条件及测序反应纯化方法和仪器操作等进行研究。
Methods:DNA sequencing and restriction endonuclease reaction was used.
方法：采用DNA测序及限制性酶切反应方法。
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