T7 and SP6 were taken as sequencing primers for forward and backward sequencing identification automatically.

对其阳性克隆扩大培养，大量提取质粒，以T7，SP6为测序引物进行全自动正、反向测序确认。

youdao

The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.

重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。

youdao

Using normal sequencing primers adjacent to multiple clone sites of plasmid as flanking primers, a novel megaprimer method was performed in one tube with two steps and 34 cycles.

采用大引物方法，利用质粒多克隆位点两侧的普通测序引物作为旁侧引物，在单个PCR管内，经2个步骤共34个循环进行定点突变。

youdao