We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.

经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。

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Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.

方法采用基因克隆、测序及生物信息学技术，分析HLA新等位基因与HLA已知基因序列的差异。

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Methods: PCR, gene cloning and successive sub cloning, DNA sequencing, prokaryotic temperature induction, etc. were used.

方法：采用PCR，克隆及连续亚克隆，序列分析，原核温度诱导表达等方法。

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Orion Genomics hopes to start pulling in revenues next year from its proprietary method of sequencing, mapping and cloning agricultural genes--technology that can be used to improve crop yield, develop pest resistance or help plants survive drought, flooding and cold.

FORBES: Yes, there is a pulse

Caves were thought to be essentially devoid of life until six years ago, when the renowned University of Colorado microbiologist Norman Pace, also an avid caver, discovered a way to identify extremophile microorganisms by cloning and sequencing unique sections of their DNA. Prior to Pace's work biologists had fruitlessly tried to cultivate these microorganisms with traditional lab techniques using nutrient-rich petri dishes.

FORBES: Magazine Article