Methods: Liquid and semi solid culture systems as well as flow cytometry were used to study the effect of PF4 on the proliferation and differentiation of CD34+ cord blood cells.
方法：用液体或半固体培养法及流式细胞术测定血小板因子4对CD34阳性脐血细胞的增殖与分化的作用。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
The hematopoietic function was evaluated with the spleen node count under microscope, semi-solid agar CFU-GM assay and peripheral blood WBC count.
通过解剖镜下计数脾结节、半固体琼脂法检测CFU -GM及常规计数外周WBC变化进行造血功能测定。
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