Therefore, the rapid, sensitive and stable semi-nested PCR are the effective methods for detecting trace bovine and sheep derived materials in feedstuff.
该技术具有快速、灵敏和结果稳定的特点，是检测饲料中痕量牛羊源成分的一种有效方法。
Compared with the conventional PCR, semi-nested PCR appeared to be more sensitive and more practicable, thus providing a reliable method to detect M. suis.
结论本试验建立的半巢式P CR诊断方法具有特异、敏感、实用等特点，为猪附红细胞体的检测提供了一种可靠的诊断方法。
Although using nested or semi-nested PCR can prominently increase PCR specificity, it really cannot efficiently amplify quick evolving region of DNA in our research of gene Fak56D.
虽然巢式和半巢式PCR等能显著提高扩增的特异性，而应用于高变异区的扩增结果仍是杂带多或涂抹严重，不能满足后续实验需要。
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