The foundation of rape selection marker and transformation methods were mainly summarizes in the paper. The present status of rapeseed genetic transformation were reviewed and the problems and envision for further study were discussed.
主要从油菜筛选标记及转化方法方面进行综述,并对油菜转基因上存在的问题及前景进行了探讨和展望。
参考来源 - 转基因油菜研究进展—《现代农业科技》—2010年第9期—龙源期刊网Optimization of genetic transformation system of“Xuegan”using PMI selection marker.
通过对影响转化的各种因素的优化实验建立了雪柑以PMI基因/甘露糖选择标记系统的高效遗传转化体系。
参考来源 - 拟南芥LEAFY及龙眼LEAFY同源基因转化雪柑研究So it is more important to establish a new genetic transformation technique without selection marker than to eliminate the selection markers from transgenic plants.
因此，建立不依赖选择标记的转基因技术比完成转基因后再剔除选择标记具有更重要的意义。
参考来源 - 用分割法建立不依赖选择标记的转基因技术·2,447,543篇论文数据，部分数据来源于NoteExpress
The results indicated that herbicide resistant gene should be used as a selection marker gene in protoplast genetic transformation of B. bassiana.
结果表明，外源基因转化球孢白僵菌原生质体时，可以以抗除草剂基因为转化筛选标记。
Therefore, it is proposed that stoma density and stomatal conductance of the lower leaves on tobacco plant be used as a selection marker for disease-resistance breeding.
因此，下部叶气孔密度和气孔导度可以作为抗病育种的选择指标。
The organization then took that knowledge and used conventional breeding techniques as well as marker-assisted selection to derive two new varieties of the crop.
然后该组织利用这种知识，并使用常规育种技术以及标志协助选育，获得两种新的作物品种。
应用推荐