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大段骨缺损；
Objective To study the difference of repairing segmental bone defect by tissue engineered bone cryopreserved by various methods.
目的探讨不同方法低温保存的组织工程骨修复节段性骨缺损的差异。
Conclusions: Primary bone lengthening were effective for the reconstruction of bone defect, this method provide a new therapeutic option to treat dia-physeal segmental bone defects.
结论：骨缺损能通过原位骨延长治疗取得成功，这对临床治疗骨缺损提供多一种治疗方法。
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