PBS is often used for diluting secondary antibodies or streptavidin-HRP conjugate.

PBS是通常用于稀释二级抗体或生物素- HRP的结合。

youdao

If there were just-- if you were trying to create a positive reaction of antigens and it showed up naturally wouldn't it create this reaction anyway in terms of your body would create antibodies like the secondary response volume to antibodies?

如果这里仅有,如果在自然条件下,尝试建立抗原的阳性反应,抗体这是否会像这样反应,以你身体为例,你的身体是否会产生,同二次免疫一样的抗体浓度

耶鲁公开课 - 生物医学工程探索课程节选

One of the things that I didn't mention before is that when you get this primary response and then the secondary response, if you looked at the antibodies that are generated during the primary response, again we're only looking at antibodies that bind to the particular antigen or vaccine that we have used for the priming.

还有一点我之前没有提及,当你获得初次免疫,以及再次免疫之后,如果你仔细观察抗体,观察那些在初次免疫中生成的抗体,这次我们同样只是观察抗体,那些与我们在初次免疫中所用到的,特定抗原或疫苗相结合的抗体

耶鲁公开课 - 生物医学工程探索课程节选