Methods: the blood samples were collected from HFRS patients between 2000 ~ 2001, and the m segment genome of hantavirus was amplified by RT-PCR.
方法：采集2000 ~ 2001年肾综合征出血热(HFRS)患者的血液标本，通过逆转录聚合酶链反应(RT - PCR)扩增汉坦病毒M片断基因。
Methods MDR1 gene expression in case of 30 leukemia and 8 healthy persons' peripheral blood have been detested by fluorescence-quantitative reverse transcription-polymerase chain reaction (RT-PCR).
方法应用荧光定量逆转录-多聚酶链反应（RT -PCR）检测了30例急性白血病患者和8例正常人外周血MDR1基因的表达。
Methods RNA from human peripheral blood mononuclear cells were extracted and target genes were amplified by RT-PCR.
方法提取人外周血单个核细胞总RNA，反转录聚合酶链反应（RT-PCR）扩增目的基因；
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