Cells were cultured at 37℃in a humidified 5%(v/v) CO_2 atmosphere for 7-9 days. When coalesce in the percentage of 70-80,the cells were put on the rocking bed which kept shaking for 18 hours(37.0℃,240 r/min).
37.0℃,5%CO_2孵箱中培养7-9 d,待培养的细胞70%-80%融合时,将培养瓶置于恒温旋转摇床上摇18 h(37.0℃,240 r/min)后,进行传代培养即得纯化的星形胶质细胞,纯化的细胞进一步运用免疫细胞化学SABC法进行鉴定。
参考来源 - 柴胡皂苷a对IL·2,447,543篇论文数据，部分数据来源于NoteExpress
Created for relaxation awesome rocking bed and chair can be enjoyed indoors or outdoors.
你能在户内外享受这些为了放松而创造出来的了不起的摇摇床和摇摇椅。
The paper expounds scouring course of rocking furnace bed in medium carbon FeMn process and application effect of magnesite-carbon brick to rocking furnace.
论述了中碳锰铁工艺线中的摇炉炉衬侵蚀过程和镁碳砖在摇炉上的应用效果。
The movable backrest plate bed is characterized in that: along with the rocking up of the movable backrest plate, the pulley pole support is inclined by the backrest plate until being supported.
本发明的特征在于随着活动靠背板的摇起，滑轮支杆随靠背板倾斜直至撑起。
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