• 摇床 - 引用次数：2

  Cells were cultured at 37℃in a humidified 5%(v/v) CO_2 atmosphere for 7-9 days. When coalesce in the percentage of 70-80,the cells were put on the rocking bed which kept shaking for 18 hours(37.0℃,240 r/min).

  37.0℃,5%CO_2孵箱中培养7-9 d,待培养的细胞70%-80%融合时,将培养瓶置于恒温旋转摇床上摇18 h(37.0℃,240 r/min)后,进行传代培养即得纯化的星形胶质细胞,纯化的细胞进一步运用免疫细胞化学SABC法进行鉴定。

  参考来源 - 柴胡皂苷a对IL

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