We inoculate cells of the third generation according to the density of 1×10~5/cm~2 ,and add the DMEM adipocyte-induced liquid contained rh-insulin 10mg/L,dexamethasone 10-6mol/L,and IBMX 0.5 mmol/L to be cultured for 2 weeks.
取传至3代的细胞按1×10~5/cm~2密度接种,加入含重组人胰岛素10mg/L、地塞米松10~(-6)mol/L、0.5mmol/L IBMX的DMEM成脂诱导液培养2周。
参考来源 - 茶黄素对兔骨髓基质干细胞向脂肪细胞诱导分化的影响·2,447,543篇论文数据,部分数据来源于NoteExpress
应用推荐