Methods The plasmid transfection mediated by liposomal transfection reagent, HPLC analysis and reverse transcription-PCR analysis were used in this study.

方法采用脂质体介导的质粒转染、流式细胞仪分析、逆转录- PCR及高效液相色谱(HPL C)分析等实验技术方法做有关分析研究。

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Under reverse microscopy, cells after transfection became round, diopter degraded, sheded easily, at the same time, cell proliferation was down-regulated.

倒置显微镜下观察见转染后见部分细胞边缘圆，折光度降低，细胞增殖减慢且易脱落；

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