diastolic synchronized coronary sinus retroperfusion 舒张期同步冠状窦逆灌注
Results76.4±19.5% of the pancreas volume expanded with antegrade perfusion, obviously surpass retroperfusion(51.1±17.7%, P<0.05). The best condition for pancreas digestion is infusion 2ml/mouse of 0.5mg/ml collagenaseXI into the pancreas, placed into 6ml of 0.5mg/ml collagenaseXI,37℃waterbath for 15min.
结果顺行灌注法胰腺膨胀比例为:76.4±19.5%明显优于逆行灌注法(51.1±17.7%,P<0.05),0.5mg/ml胶原酶XI 2ml/mouse灌注,置于6ml 0.5mg/ml胶原酶XI中,37℃水浴静置消化15min,为最佳消化条件。
参考来源 - 小鼠胰岛分离纯化和不同部位移植的研究·2,447,543篇论文数据,部分数据来源于NoteExpress
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