Tamino main shape of the three categories of music genres, respectively specific analysis.
主要以塑造塔米诺的三种音乐体裁为类别，进行分别具体分析。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
The titer of the antiserum was respectively 1:256 by agarose double-diffusion analysis and it was 1:12800 by indirect ELISA, specific antisera against the fusion protein were prepared.
琼脂糖双向扩散检测显示抗血清的效价1：25 6，间接ELISA测得的效价1：12 800，结果显示获得了融合蛋白的特异性抗血清。
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