Now the plasmid started to do its thing, which is replicate and the gene gets transcribed.

那么质粒就可以开始进行复制,基因得以转录

耶鲁公开课 - 生物医学工程探索课程节选

One of the properties that you would like a plasmid to have is you'd like for cells to be able to replicate it, to make more copies of it.

所以你所希望质粒具备的一个属性,就是得能让细胞复制它,制造出更多的拷贝

耶鲁公开课 - 生物医学工程探索课程节选

Take a plasmid, cut it open, insert a gene that we want into the plasmid, and then put that plasmid in a host cell, and let the host replicate it.

取一个质粒,把它切开,把目标基因放进去,然后把这个质粒植入到宿主细胞,让宿主来进行复制

耶鲁公开课 - 生物医学工程探索课程节选