...:生物大分子的天然构象遭到破坏导致其生物活性丧失的现象。蛋白质在受到光照,热,有机溶济以及一些变性济的作用时,次级键受到破坏,导致天然构象的破坏,使蛋白质的生物活性丧失。复性(renaturation):在一定的条件下,变性的生物大分子恢复成具有生物活性的天然构象的现象。
基于938个网页-相关网页
protein renaturation 蛋白再生 ; 蛋白复性
denaturation renaturation 核酸的变性与复性
denaturation and renaturation 变性与复性
renaturation dynamics 复性动力学
optimum renaturation temperature 最适复性温度
renaturation fo protein 蛋白质复性
Immobilization Renaturation 固定化复性
renaturation of protein 蛋白质的变性恢复
IB solubilized in the presence of 8mol/L urea were diluted with renaturation solutions so that folding process initiated. Concentrated IL-3 were digested with thrombin and authentic rhIL-3 released.
超声破碎细菌细胞得包涵体,经洗涤处理可使包涵体纯度达90%,用8mol/L尿素变性溶解包涵体沉淀后直接稀释复性,再超滤浓缩、凝血酶消化,释放天然型rhIL-3。
参考来源 - 大肠杆菌表达的人重组ILThe activity of expressed protein after renaturation had recovered and further confirmed by lymphocyte proliferation assay in vitro.
ELISA鉴定复性后的蛋白已恢复活性,并能在体外刺激外周血单个核细胞的增殖。
参考来源 - 人源鼻咽癌双价抗独特型抗体疫苗的制备及活性鉴定·2,447,543篇论文数据,部分数据来源于NoteExpress
应用推荐