Real-time (RT) application development distinguishes itself from general-purpose application development by imposing time restrictions on parts of the runtime behavior.

实时（RT）应用程序开发与通用应用程序开发的差异在于前者对部分运行时行为强加了时间限制。

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Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.

目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。

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We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.

我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证，证实了基因芯片结果的可靠性。

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Real-Time Polymerase Chain Reaction (RT-PCR) uses nanoscaled fluorescent probes that allow the tracking of the progress of the amplification from the start.

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