Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes.

通过表达谱芯片检测药物作用后的基因表达差异，对差异表达基因进行生物信息学研究并结合实时荧光定量pcr分析。

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Objective To increase the sensitivity of quantitative detection of gene transcripts in peripheral blood using real time RT PCR, two specimen processing methods were compared.

目的选择合理的标本处理方法，提高外周血基因转录本检测的灵敏度。

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We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.

我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证，证实了基因芯片结果的可靠性。

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Real-Time Polymerase Chain Reaction (RT-PCR) uses nanoscaled fluorescent probes that allow the tracking of the progress of the amplification from the start.

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