The method of isolating genomic DNA and RAPD primer screening of were studied. The results are as follows;
以毛豹皮樟叶片为材料,对其DNA提取方法和RAPD引物的筛选进行了研究。
Among 109 loci revealed by 20 RAPD primers, only primer S303 and S4 showed 4 polymorphic loci. The percent of polymorphic loci (p) was only 3.7%.
所分析的20个随机引物共扩增出了109条带,但是只有引物s303、S4出现4个多态位点,多态性位点所占的比例(P)只有3.7%。
It was found these 23 fragments, which were cloned and sequenced after randomly selected, were the products from their corresponding RAPD primer amplification.
通过对随机挑选的23个片段进行克隆与测序,结果发现23个片段都是对应引物的RAPD扩增产物。
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