Genetic difference of 18 processing and 2 fresh apple varieties were evaluated with 12 simple sequence repeats (SSR) primer pairs.
利用12对微卫星（SSR）标记，研究了18个苹果加工品种和2个鲜食品种的遗传差异。
In total, 272 loci were amplified with 4 primer pairs, of which 269 were polymorphic and the percentage of polymorphic loci was 98.89%.
结果表明，4对引物共扩增得到272个位点，其中269个多态位点，总的多态位点比例为98.89%。
The following work were done in this study: (1)TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK;
主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；
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