A primary culture of myoblasts may be prepared by excising skeletal muscle tissue from 10 to 30 day old rat embryos.
制备成肌细胞的初级培养物，可以将10-30天的大鼠胚胎骨骼肌组织剪切下来。
The primary culture cells of rabbit skin tissue could be obtained by explant culture too.
用组织块直接培养法，也可以得到皮肤的原代培养细胞。
Artery and vein were removed and mesenchymal tissue was taken out. The primary cells were performed culture and purification with collagenase digestion method to obtain the adherent cells.
无菌条件下取正常足月剖腹产的脐带近胎儿段，剔除动静脉，取出其中的间质组织，胶原酶消化法原代培养细胞进行培养和纯化获得贴壁细胞。
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