AIM: To establish the mutant of coding calcium binding fragment of the 13th exon of human thrombospondin-1 (TSP-1) gene with polymerase chain reaction (PCR) site directed mutagenesis technology.
目的：利用聚合酶链反应定点突变技术构建人血小板反应素1基因第13外显子编码钙结合域突变体。
Rifampicin cannot bind to the mutant RNA polymerase and so is unable to block the initiation of transcription.
利福平不能结合到突变的RNA聚合酶上，因此不能阻断转录的起始。
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