TR3 is an immediate-early response gene whose expression is rapidly induced by a variety of growth stimuli, including serum, phorbol ester and growth factors etc.
TR3是一种立早基因,可以被血清、佛波酯和各种生长因子等诱导表达。
参考来源 - TR3负调控转录辅激活因子p300的机制PBMC was isolated by Ficoll desity gradient centrifugation methods first, then the PBMC was stimulated 5 hours by phorbol ester and calcium ionophore. The cells were stained by CD4-FITC and anti IL-17-PE, and CD4+IL-17+T cell was detected by Flow Cytometry.
Ficoll密度梯度离心法分离外周血单个核细胞(PBMC),用佛波酯和钙离子载体刺激5小时后,抗CD4-FITC和抗IL-17-PE标记,流式细胞仪检测CD4+IL-17+T细胞的比例。
参考来源 - 中国汉族人类风湿关节炎易感基因及Th17细胞的研究·2,447,543篇论文数据，部分数据来源于NoteExpress
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