The selection of moving phase, gradient eluting procedure and line relation of sampling concentration with peak height were discussed.
研究了流动相的选择，梯度淋洗程序的最佳化以及进样浓度与峰高的线性关系。
Gauss interpolation realized the fine focusing nearby the peak of focusing curve based on the gradient energy function.
利用梯度平方函数高斯插值实现在峰值附近的精确调焦。
The gradient elution is reasonable when the retention time is 2.3 times after the appearance of prominent peak with initial mobile phase acetonitrile-water (40:60).
用初始流动相乙腈-水(40：6 0)洗脱至主峰出峰保留时间的2.3倍时开始进行梯度洗脱比较合理。
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