Methods The attachment rate of PDLCs was analyzed by MTT and the morphology of PDLCs was observed by florescence microscope.

方法 MTT法分析牙周韧带细胞在商用纯钛表面的早期粘附 ，采用落射荧光观察细胞的粘附形态。

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RESULTS: PDLCs adhered and proliferated better in MTA group than in amalgam group in microscope, while in IRM group PDLCs showed no proliferation.

结果：光镜显示，人PDLC在空白组，MTA组胶原凝胶上黏附伸展良好，银汞合金组伸展较好，IRM组细胞不伸展。

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Commasie brilliant blue staining was used to study the total protein content and transmission electron microscopy was used to study the ultrastructure of PDLCs.

通过考马斯亮蓝法测定牙周膜细胞总蛋白含量，透射电子显微镜观察细胞超微结构的变化。

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