应用人血小板衍生生长因子(PDGF-BB)作为自噬诱导剂,3-甲基腺嘌呤(3-MA)作为自噬的抑制剂,在mi R-30a-5p作用下通过调节自噬来观察mi RNA-30a-5p对HSCs激活的影响。
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AIM: to investigate the effects of platelet-derived growth factor BB (PDGF-BB) on vasoconstriction of the vascular vessels.
目的：观察血小板衍生生长因子- BB (PDGF - BB)对大鼠主动脉血管环的收缩作用。
The cell culture liquid containing PDGF-BB was active and could be neutralized by the polyclonal antibody of human PDGF-BB.
MTT方法较为稳定，PDGF— BB细胞培养液具有一定活性，并能被抗人PDGF-BB 多克隆抗体中和。
The contents of PDGF-BB were obviously higher than those of control group (all P<0.05), and they gradually reduced with time.
而PDGF-BB含量也明显高于对照组（P均＜0．05），但随术后时间延长逐渐恢复降低。
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